Cell responses to xenobiotics: comparison of MCF7 multi-drug- and mussel blood cell multi-xenobiotic-defense mechanisms.
Identifieur interne : 001740 ( France/Analysis ); précédent : 001739; suivant : 001741Cell responses to xenobiotics: comparison of MCF7 multi-drug- and mussel blood cell multi-xenobiotic-defense mechanisms.
Auteurs : Matthieu Marin [France] ; Hélène Legros ; Agnès Poret ; François Leboulenger ; Frank Le FollSource :
- Marine environmental research [ 0141-1136 ]
English descriptors
- KwdEn :
- Analysis of Variance, Animals, Biological Transport (drug effects), Bivalvia (metabolism), Cell Line, Tumor, Colorimetry, Doxorubicin (pharmacology), Drug Resistance, Multiple (drug effects), Fluoresceins (metabolism), Fluorometry, Hemocytes (metabolism), Humans, P-Glycoprotein (metabolism), Tetrazolium Salts, Thiazoles, Verapamil (pharmacology), Vincristine (pharmacology), Xenobiotics (pharmacology).
- MESH :
- chemical , metabolism : Fluoresceins, P-Glycoprotein.
- chemical , pharmacology : Doxorubicin, Verapamil, Vincristine, Xenobiotics.
- drug effects : Biological Transport, Drug Resistance, Multiple.
- metabolism : Bivalvia, Hemocytes.
- Analysis of Variance, Animals, Cell Line, Tumor, Colorimetry, Fluorometry, Humans, Tetrazolium Salts, Thiazoles.
Abstract
Multi-drug resistance (MDR) in MCF7 breast cancer cells and multi-xenobiotic resistance (MXR) in mussel (Mytilus edulis) blood cells (MBC) are well known mechanisms that contribute to the decrease in intracellular concentrations of many unrelated but cytotoxic compounds. In the present work, we have carried out comparative investigations of the MDR/MXR protective mechanisms using a rapid colorimetric assay for cell viability and calcein accumulation for MDR/MXR activities. These studies were performed using cultured MCF7 and MBC before and after in vitro exposure to xenobiotics. Our results indicate that a 5-day exposure to doxorubicin or vincristine decreased calcein accumulation in MBC which is consistent with an induction of multi-xenobiotic resistance. The increase in calcein accumulation provoked by 1-h treatment with 50 microM verapamil was much lower in MBC when compared to the P-glycoprotein overexpressing MCF7 cell line. We conclude that such microplate assays could be used in primary cultures of MBC to estimate the effects of various chemicals on MXR activity.
DOI: 10.1016/j.marenvres.2004.03.016
PubMed: 15178034
Affiliations:
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pubmed:15178034Le document en format XML
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first="Hélène" last="Legros">Hélène Legros</name></author><author><name sortKey="Poret, Agnes" sort="Poret, Agnes" uniqKey="Poret A" first="Agnès" last="Poret">Agnès Poret</name></author><author><name sortKey="Leboulenger, Francois" sort="Leboulenger, Francois" uniqKey="Leboulenger F" first="François" last="Leboulenger">François Leboulenger</name></author><author><name sortKey="Le Foll, Frank" sort="Le Foll, Frank" uniqKey="Le Foll F" first="Frank" last="Le Foll">Frank Le Foll</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="????"><PubDate><MedlineDate>2004 Aug-Dec</MedlineDate></PubDate></date><idno type="RBID">pubmed:15178034</idno><idno type="pmid">15178034</idno><idno type="doi">10.1016/j.marenvres.2004.03.016</idno><idno type="wicri:Area/PubMed/Corpus">000335</idno><idno type="wicri:Area/PubMed/Curation">000335</idno><idno type="wicri:Area/PubMed/Checkpoint">000335</idno><idno type="wicri:Area/Ncbi/Merge">000104</idno><idno 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UPRES-EA 3222, IFRMP 23, University of Le Havre, BP 540 76058 Le Havre</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Legros, Helene" sort="Legros, Helene" uniqKey="Legros H" first="Hélène" last="Legros">Hélène Legros</name></author><author><name sortKey="Poret, Agnes" sort="Poret, Agnes" uniqKey="Poret A" first="Agnès" last="Poret">Agnès Poret</name></author><author><name sortKey="Leboulenger, Francois" sort="Leboulenger, Francois" uniqKey="Leboulenger F" first="François" last="Leboulenger">François Leboulenger</name></author><author><name sortKey="Le Foll, Frank" sort="Le Foll, Frank" uniqKey="Le Foll F" first="Frank" last="Le Foll">Frank Le Foll</name></author></analytic><series><title level="j">Marine environmental research</title><idno type="ISSN">0141-1136</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Analysis of Variance</term><term>Animals</term><term>Biological Transport (drug effects)</term><term>Bivalvia (metabolism)</term><term>Cell Line, Tumor</term><term>Colorimetry</term><term>Doxorubicin (pharmacology)</term><term>Drug Resistance, Multiple (drug effects)</term><term>Fluoresceins (metabolism)</term><term>Fluorometry</term><term>Hemocytes (metabolism)</term><term>Humans</term><term>P-Glycoprotein (metabolism)</term><term>Tetrazolium Salts</term><term>Thiazoles</term><term>Verapamil (pharmacology)</term><term>Vincristine (pharmacology)</term><term>Xenobiotics (pharmacology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fluoresceins</term><term>P-Glycoprotein</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Doxorubicin</term><term>Verapamil</term><term>Vincristine</term><term>Xenobiotics</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Biological Transport</term><term>Drug Resistance, Multiple</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Bivalvia</term><term>Hemocytes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Analysis of Variance</term><term>Animals</term><term>Cell Line, Tumor</term><term>Colorimetry</term><term>Fluorometry</term><term>Humans</term><term>Tetrazolium Salts</term><term>Thiazoles</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Multi-drug resistance (MDR) in MCF7 breast cancer cells and multi-xenobiotic resistance (MXR) in mussel (Mytilus edulis) blood cells (MBC) are well known mechanisms that contribute to the decrease in intracellular concentrations of many unrelated but cytotoxic compounds. In the present work, we have carried out comparative investigations of the MDR/MXR protective mechanisms using a rapid colorimetric assay for cell viability and calcein accumulation for MDR/MXR activities. These studies were performed using cultured MCF7 and MBC before and after in vitro exposure to xenobiotics. Our results indicate that a 5-day exposure to doxorubicin or vincristine decreased calcein accumulation in MBC which is consistent with an induction of multi-xenobiotic resistance. The increase in calcein accumulation provoked by 1-h treatment with 50 microM verapamil was much lower in MBC when compared to the P-glycoprotein overexpressing MCF7 cell line. We conclude that such microplate assays could be used in primary cultures of MBC to estimate the effects of various chemicals on MXR activity.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Haute-Normandie</li><li>Région Normandie</li></region><settlement><li>Le Havre</li></settlement></list><tree><noCountry><name sortKey="Le Foll, Frank" sort="Le Foll, Frank" uniqKey="Le Foll F" first="Frank" last="Le Foll">Frank Le Foll</name><name sortKey="Leboulenger, Francois" sort="Leboulenger, Francois" uniqKey="Leboulenger F" first="François" last="Leboulenger">François Leboulenger</name><name sortKey="Legros, Helene" sort="Legros, Helene" uniqKey="Legros H" first="Hélène" last="Legros">Hélène Legros</name><name sortKey="Poret, Agnes" sort="Poret, Agnes" uniqKey="Poret A" first="Agnès" last="Poret">Agnès Poret</name></noCountry><country name="France"><region name="Région Normandie"><name sortKey="Marin, Matthieu" sort="Marin, Matthieu" uniqKey="Marin M" first="Matthieu" last="Marin">Matthieu Marin</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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